Welcome to Nature Protocols
Protocols Network Submission System
This system is for uploading protocols to the open-access Protocols Network part of Nature Protocols. Protocols uploaded here are not peer-reviewed or extensively edited, but are made live on the site soon after uploading to allow timely access to all.
View tips on how to prepare and upload your protocol [PDF (501K)]. See below for examples of content.
When you are ready to submit your protocol, please log in using your nature.com username and password or register for an account on nature.com. If you encounter any problems, please contact us at protocols@nature.com.
Here are some examples of Network Protocols
CELL AND TISSUE CULTURE / IMAGING
This is a protocol for the isolation and expansion of neural stem cells from tissue dissections of the post-natal mouse brain. Microscopical analysis and cryopreservation steps are also described.
SYNTHETIC CHEMISTRY
Synthesis of N-Boc pyrrole 2-ethyl ester
N-Boc pyrrole 2-ethyl ester is a key intermediate in the Donohue synthesis of omuralide described in the Nature Protocol: The partial reduction of electron-deficient pyrroles: procedures describing both Birch (Li/NH3) and ammonia-free (Li/DBB) conditions
COMPUTATIONAL AND THEORETICAL BIOLOGY
AFPredictor is a surface-based pattern detection algorithm that can be used to investigate the structural characteristics of antifreeze/ice-structuring proteins.
CELL AND TISSUE CULTURE
Rat Chromaffin cells primary culture
Cultured chromaffin cells are a well established model for neurosecretion studies. This protocol describes a method for the isolation, culture and quality assessment of chromaffin cells from rat adrenal medulla that results in improved yields and cell survival.
BIOCHEMISTRY AND PROTEIN ANALYSIS
Western blot analysis using Infrared Imaging
This protocol describes Western blot detection of proteins from sub-cellular fractionated samples using the Odyssey Infrared Imaging System.
